Assessment of Seed Germination of Moringa peregrina under Drought and Salinity Stress and its Cardinal Germination Temperatures in Laboratory Environment

Document Type : Original Article


1 Assistant professor, Faculty of Natural Resources, University of Jiroft

2 Ph.D. student of seed science and technology, College of Agriculture and Natural Resources, University of Tehran

3 PhD Student, Faculty of Natural Resources, University of Tehran

4 Associate Professor, Faculty of Natural Resource, University of Tehran

5 Assistant Professor, Faculty of Natural Resources, University of Jiroft, Kerman, Iran



The effect of different temperatures on germination was assessed in a laboratory study in the framework of complete randomized design with five repetitions to determine the specific temperature of Moringa peregrina seeds. Evaluation of germination response was conducted at constant temperatures of 5, 10, 15, 20, 25, 30, 35, 40 and 45 °C. Cardinal germination temperatures were fitted using three models, including beta, segmented and dent-like. Optimum and maximum temperatures of germination were calculated 17, 25-30 and 47 °C, respectively, based on the dent-like model, which was identified as the best model using statistical indicators. Then, to investigate germination and seedling growth response of Moringa peregrina toward different levels of salinity and drought stress at an optimum temperature, another test was conducted. In this experiment, seed germination was assessed in four levels of salinity and drought with the osmotic potential of 0, -4, -8 and -12 bar. The results indicated that seed germination speed and percentage were decreased due to drought and salinity stress. Generally, seed germination of Moringa peregrina was more sensitive to drought stress than to salinity stress.


  1. Adam, N. R., Dierig, D. A., Coffelt, T. A., Wintermeyer, M. J., Mackey, B. E., Wall, G. W., 2007. Cardinal temperatures for germination and early growth of two Lesquerella species. Industrial Crops and Products, 25(1), 24-33.
  2. Al-Dabbas, M.M., 2017. Antioxidant activity of different extracts from the aerial part of Moringa peregrina (Forssk.) Fiori, from Jordan. Pakistan journal of pharmaceutical sciences, 30(6).
  3. Amiri, MJ., Eslamian, SS., 2010. Investigation of climate change in Iran. J Environ Sci Technol. 3; 208–216.
  4. Ashraf, M., Foolad MR., 2005. Pre-sowing seed treatment-a shotgun approach to improve germination, plant growth, and crop yield under saline and non-saline conditions. Advances in Agronomy 88; 223-271.
  5. Bidgoly, R.O., Balouchi, H., Soltani, E., Moradi, A., 2018. Effect of temperature and water potential on Carthamus tinctorius L. seed germination: Quantification of the cardinal temperatures and modeling using hydrothermal time. Industrial Crops and Products, 113; 121-127.
  6. Bradford, K.J.,2002. Applications of hydrothermal time to quantifying and modeling seed germination and dormancy. Weed Science, 50(2); 248-260.
  7. Brindle, M., and K. Jensen., 2005. Effect of temperature on dormancy and germination of Eupatorium L. achenes. Seed Sci Res, 15; 143-151.
  8. Bybordi, A., Tabatabaei, J., 2009. Effect of salinity stress on germination and seedling properties in canola cultivars (Brassica napus L.). Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 37(2).
  9. Canter, P.H., Thomas, H., Ernst, E., 2005. Bringing medicinal plants into cultivation: opportunities and challenges for biotechnology. TRENDS in Biotechnology, 23(4); 180-18.
  10. Colbach, N., Chauvel, B., Dürr, , Richard, G., 2002. Effect of environmental conditions on Alopecurus myosuroides germination. I. Effect of temperature and light. Weed Research, 42(3); 210-221.
  11. Coskun, D., Britto, D.T., Huynh, W.Q., Kronzucker, H.J., 2016. The role of silicon in higher plants under salinity and drought stress. Frontiers in plant science, 7, 1072.
  12. Dodd, G.L., Donovan, L.A.,1999. Water potential and ionic effects on germination and seedling growth of two cold desert shrubs. American Journal of Botany, 86(8); 1146-1153.
  13. Fang, F., Zhang, C., Wei, S., Huang, H., Liu, W., 2012. Factors affecting Tausch’s Goatgrass (Aegilops tauschii Coss.) seed germination and seedling emergence. J Agri Sci. 4; 114–121.
  14. Garg, G.,2010. Response in germination and seedling growth in Phaseolus mungo under salt and drought stress. Journal of environmental biology.Academy of Environmental Biology, India, 31(3); 264.
  15. Gohari, AR., Eslamian, S., Abedi-Koupaei, J., Massah Bavani, AR., Wang, D., Madani, K., 2013. Climate change impacts on crop
  16. Hardegree, S.P.,2006. Predicting germination response to temperature. I. Cardinal-temperature models and subpopulation-specific regression. Annals of Botany, 97(6); 1115-1125.
  17. Hashemi, A,.Tavakkol Afshari, R., Tabrizi, L.,2016. investigation of germination properties and important temperatures of Plantago ovate seed, Journal of Iran agricultural plants science, 47(1); 1-7.
  18. Ibrahim, EA., 2016. Seed priming to alleviate salinity stress in germinating seeds. Journal of Plant Physiology 192; 38-46.
  19. Jame, Y., Cutforth, H.,2004. Simulating the effects of temperature and seeding depth on germination and emergence of spring wheat. Agricultural and Forest Meteorology, 124(3); 207-218.
  20. Karavani, B., Tavakol afshari, R., Majnoon hoseini, N., Moosavi, S.A.,2014. Investigation of seed germination peroperties of Scrophularia Striata under droght and salinity stressess in different temperatures. Journal of Iran agricultural plants science, 45(2); 265-275.
  21. Kaufman, R., Barlyn, E., Michel, N.,1973. The osmotic potential of polyethylene glycol 6000. Plant Physiology 51; 914-916.
  22. Khan, M.A., Gulzar, S.,2003.Germination responses of Sporobolus ioclados: a saline desert grass. Journal of Arid Environments, 53(3); 387-394.
  23. Laghmouchi, Y., Belmehdi, O., Bouyahya, A., Senhaji, N.S., Abrini, J., 2017. Effect of temperature, salt stress and pH on seed germination of medicinal plant Origanum compactum. Biocatalysis and agricultural biotechnology, 10; 156-160.
  24. Mehdinejad, M.H., Bina, B., Nikaeen, M., Movahedian-Attar, H.,2009. Effectiveness of Moringa Oliefera Coagulant Protein and Chitosan as natural coagulant aids in removal of colloidal particles and bacteria from turbid water, Journal of Gorgan university of Medical Science, 11;66-69.
  25. Rauf, M., Munir, M., ul Hassan, M., Ahmad, M., Afzal, M.,2007. Performance of wheat genotypes under osmotic stress at germination and early seedling growth stage. African Journal of Biotechnology, 6(8).
  26. Raziei, T., Saghafian, B., Paulo, A.A., Pereira, L.S. & Bordi, I.,2009. Spatial patterns and temporal variability of drought in western Iran. Water Resources Management, 23(3); 439-455.
  27. Saha, P., Raychaudhuri, S., Mishra, D., Chakraborty, A., & Sudarshan, M., 2008. Role of trace elements in somatic embryogenesis–A PIXE study. Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, 266(6); 918-920.
  28. Schelin, M., Tigabu, M., Eriksson, I., Sawadogo, L., & Oden, P. C., 2003. Effects of scarification, gibberellic acid and dry heat treatments on the germination of Balanites aegyptiaca seeds from the Sudanian savanna in Burkina Faso. Seed Science and Technology, 31(3); 605-617.
  29. Sanchez- Martin, J., Ghebremichael, k. and Beltrn-Heredia, J.,2010. Comparison of sigle-step and two-step purified coagulants from Moringa oliefera seed for turbidity and DOC removal. Bioresource technology. 101; 6259- 6261.
  30. Seied sharifi, r.,2007. the effect of salinity on germination indices Silybum Marianum.The third Conference of Medicinal Plants, P. 207-212.
  31. Soltani, A., Sinclair, TR,. 2012. Modeling physiology of crop development, growth and yield. Oxfordshire: CABI Press; p.
  32. Soltani, A., Maddah, V.,2010. Simple, applied programs for education and research in agronomy. Shahid Beheshti University Press.
  33. Soltani, A., Galeshi, S., Zeinali, E., Latifi, N.,2002. Germination, seed reserve utilization and seedling growth of chickpea as affected by salinity and seed size. Seed Science and Technology, 30; 51-60.
  34. Stadtlander, T., Becker, K., 2017. Proximate Composition, Amino and Fatty Acid Profiles and Element Compositions of Four Different Moringa Journal of Agricultural Science, 9(7); 46.
  35. Torabi, B., Soltani, E., Archontoulis, S.V., Rabii, A., 2016. Temperature and water potential effects on Carthamus tinctorius L. seed germination: measurements and modeling using hydrothermal and multiplicative approaches. Brazilian Journal of Botany, 39(2); 427-436.
  36. Turhan, H., Ayaz, C.,2004. Effect of salinity on seedling emergence and growth of sunflower (Helianthus annuus L.) cultivars. Int. J. Agric. Biol, 6(1); 149-152.
  37. Zeinali, e., Soltani, a., & Galeshi, s.,2002. Components of seed germination response to salt stress in Brassica napus. Journal of Agricultural Sciences Iran. 32; 137-145.